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新西兰nursing代写 护理实验
2020-02-09 03:55
上述实验所用材料包括米尺、6支试管、试管架、记号笔、试管架、烧杯、本尼迪克试剂、热板。分别从肠系膜动脉、肝门静脉、肝静脉采集空腹血清,餐后(餐后)分别从肠系膜动脉、肝门静脉、肝静脉采集空腹血清。该试验在性质上不是定量的,因此被用来比较血清样品中葡萄糖的相对数量,通过观察反应发生的速度和颜色的变化程度。血清样本置于试管中,用本尼迪克特溶液加热。然后准备了一个水浴。同时用米尺在距离底部1cm和2cm处划去6根管,以表示实验中所用血清和试剂的体积。所有的试管都根据它们来自的血管和食用前后的适当样本进行了标记。在进食前和进食后,所有的试管都被填满到所需的水平。每管都相应地在第一个标记上标记了血清。然后将本尼迪克特试剂添加到第二个标记上。然后,三个餐后样本同时在水浴中加热,3分钟后记录颜色变化的时间。三个空腹样本也被放置在相同的实验条件下。本尼迪克特试剂由蓝色(阴性)变为黄色,橙色,然后变为红色,因为葡萄糖在样品中的含量增加了。所有的实验程序完成后,所使用的本尼迪克试剂被放入适当的废瓶中。新西兰nursing代写 护理实验
The materials used in the above experiment included a metric ruler, 6 test tubes, test tube rack, marking pen, test tube holder, beaker, Benedict’s reagent, and hot plate. Fasting serum samples were collected from the mesenteric arteries, hepatic portal vein, and hepatic vein while postprandial samples (after eating) were collected from the mesenteric arteries, hepatic portal vein, and hepatic vein.The test was not quantitative in nature and was thus used to compare the relative quantities of glucose in serum samples by observing how quickly the reaction occurred and degree of change in color. Serum samples were placed in tubes and were heated by Benedict’s solution. A water bath was then prepared. At the same time a metric ruler was used to mark off 6 tubes at 1 cm and 2 cm from the bottom to indicate the volume of serum and reagent which was to be used in the experiment. All the tubes were marked according to the blood vessels they came from and from the appropriate samples before and after eating. All the tubes were filled to its required levels before and after eating. Each tube was labelled accordingly with the serum on the first mark. Benedict reagent was then added to the second mark. The three postprandial samples were then simultaneously heat in a water bath and the time taken for the color changes were recorded after 3 minutes. The three fasting samples were also placed under the same experimental conditions. Benedict reagent changes from blue (negative) to yellow, orange then to red as glucose were noted as glucose levels increased in the samples. The Benedicts reagent used was then disposed in the appropriate waste jar after all experimental procedures were carried out.
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